\( \require{mhchem} \)
The Sakaguchi test is a chemical test used to detect presence of arginine in proteins. It is named after the Japanese food scientist and organic chemist, Shoyo Sakaguchi (1900–1995) who described the test in 1925.[1] The Sakaguchi reagent used in the test consists of 1-Naphthol and a drop of sodium hypobromite. The guanidino (–C group in arginine reacts with the Sakaguchi reagent to form a red-coloured complex.[2][3][4][5]
References
Sakaguchi, Shoyo (April 1925). "Über eine neue Farbenreaktion von protein und arginin" [About a new colorimetric reaction of protein and arginine]. Journal of Biochemistry (in German). Oxford University Press; Japanese Biochemical Society. 5: 25–31. doi:10.1093/oxfordjournals.jbchem.a128359.
Chatterjea (1 January 2004). Textbook of Biochemistry for Dental/Nursing/Pharmacy Students. Jaypee Brothers Publishers. p. 51. ISBN 978-81-8061-204-6.
R.A. Joshi (2006). Question Bank of Biochemistry. New Age International. p. 64. ISBN 978-81-224-1736-4.
Dandekar (1 January 2004). Practicals And Viva In Medical Biochemistry. Elsevier India. p. 28. ISBN 978-81-8147-025-6.
Joshi, Rashmi A.; Marks, Victor (30 June 2004). Guide to Bio-chemistry. B. Jain Publishers. p. 74. ISBN 9788180560538.
vte
Analytical reagents and tests
Metals
Aluminon Xylenol orange
Sugars, fats, and proteins
Sugars & starches
Benedict's reagent (reducing sugars etc) Bial's test (pentoses) Aniline acetate test (pentoses) Starch indicator Molisch's test (carbs)
Proteins & amino acids
Biuret test (proteins) Bicinchoninic acid assay (concentration) Bradford protein assay (concentration) Lowry protein assay (total protein) Pierce Protein Assay (total protein) Tollens' reagent (aldehyde & ketones) Fehling's solution (aldehyde & ketones) Van Slyke determination (amino acids) Sakaguchi test (arginine)
Fats
Sudan stain (lipids)
Alcohols
Benedict's reagent (aldehyde & ketones etc) Denigés' reagent Lucas' reagent (types of alcohols)
Drugs
Dille–Koppanyi reagent (barbiturates etc) Ehrlich's reagent (indoles, etc) Froehde reagent (opioids) Gallic acid reagent (drug precursor) Liebermann reagent (street drugs) Mandelin reagent (ketamine) Marquis reagent (MDMA, opiates, etc) Mecke reagent Drug checking Simon's reagent Zimmermann reagent (Benzodiazepines) Zwikker reagent (barbiturates) Salicylate testing Folin's reagent
Other
Dragendorff's reagent (alkaloids) Fenton's reagent (creates free radicals) Folin–Ciocalteu reagent (antioxidants) Murexide test (caffeine etc) Melzer's reagent (fungi) Marquis reagent (various) Nitrate test Nitrite test Luminol (blood) Pesticide detection kit Fecal coliform detection Prion detection kit
Hellenica World - Scientific Library
Retrieved from "http://en.wikipedia.org/"
All text is available under the terms of the GNU Free Documentation License
